St John Ambulance Australia Victoria Incorporated is part of a nationally federated system, St John Ambulance Australia.

In Victoria the St John role is to:

• Provide First Aid at public and sporting events;
• Provide First Aid in civil emergencies under Victoria's Emergency Management system;
• Conduct workplace and public First Aid Training courses;
• Sell First Aid Kits, components and manuals;
• Provide services to those in the community who are aged, infirmed or isolated under the Commonwealth Government's Friends for Older People scheme and the State Government's Visiting Friends program;
• Provide support to the St John Eye Hospital in Jerusalem; and
• Uphold the traditions, objectives and ideals of the Order of St John.